abstract：:In this short review, we summarize our work on the role of members of the FXYD protein family as tissue-specific modulators of Na, K-ATPase. FXYD1 or phospholemman, mainly expressed in heart and skeletal muscle increases the apparent affinity for intracellular Na(+) of Na, K-ATPase and may thus be important for approp...

journal_title：Journal of bioenergetics and biomembranes

authors： Geering K

更新日期：2005-12-01 00:00:00

abstract：:We summarize our current view of the reaction mechanism in F(1)-ATPase as it has emerged from experiment, theory, and computational studies over the last several years. ATP catalysis in the catalytic binding pockets of F(1) takes place without the release of any significant free energy and is efficiently driven by the...

journal_title：Journal of bioenergetics and biomembranes

authors： Dittrich M,Schulten K

更新日期：2005-12-01 00:00:00

abstract：:The ATP-binding cassette (ABC) family of membrane transport proteins is the largest class of transporters in humans (48 members). The majority of ABC transporters function at the cell surface. Therefore, defective folding and trafficking of the protein to the cell surface can lead to serious health problems. The class...

journal_title：Journal of bioenergetics and biomembranes

authors： Loo TW,Bartlett MC,Clarke DM

更新日期：2005-12-01 00:00:00

abstract：:Relevant Ca(2+) pools and fluxes in H9c2 cells have been studied using fluorescent indicators and Ca(2+)-mobilizing agents. Vasopressin produced a cytoplasmic Ca(2+) peak with half-maximal effective concentration of 6 nM, whereas thapsigargin-induced Ca(2+) increase showed half-maximal effect at 3 nM. Depolarization o...

journal_title：Journal of bioenergetics and biomembranes

authors： Lax A,Soler F,Fernández-Belda F

更新日期：2005-08-01 00:00:00

abstract：:Research on VDAC has accelerated as evidence grows of its importance in mitochondrial function and in apoptosis. New investigators entering the field are often confounded by the VDAC literature and its many apparent conflicts and contradictions. This review is an effort to shed light on the situation and identify reli...

journal_title：Journal of bioenergetics and biomembranes

authors： Rostovtseva TK,Tan W,Colombini M

更新日期：2005-06-01 00:00:00

abstract：:Evidence suggests membrane bound F(1)F(0)-ATPase complexes form stable associations such that dimers can be retrieved from detergent lysates of mitochondria isolated from a range of sources including algae, higher plants, yeast and bovine heart, and plant chloroplasts. The physiological relevance of these interactions...

journal_title：Journal of bioenergetics and biomembranes

authors： Gavin PD,Prescott M,Devenish RJ

更新日期：2005-04-01 00:00:00

abstract：:The mitochondrial ATP synthase is a membrane protein complex which couples the proton gradient across the mitochondrial inner membrane to the synthesis of ATP from ADP+Pi. The complex is composed of essential subunits for its motor functions and supernumerary subunits, the roles of which remain to be elucidated. Subun...

journal_title：Journal of bioenergetics and biomembranes

authors： Hong S,Pedersen PL

更新日期：2004-12-01 00:00:00

abstract：:Utilizing cysteine scanning mutagenesis, with functional Cys-less citrate transport protein (CTP) serving as the starting template, we previously demonstrated that four single-Cys mutants located in transmembrane domains III and IV, rendered the CTP nonfunctional. The present investigations assess and quantify the sec...

journal_title：Journal of bioenergetics and biomembranes

authors： Cascio M,Mayor JA,Kaplan RS

更新日期：2004-10-01 00:00:00

abstract：:At present there is a poor understanding of the events that lead up to neuronal apoptosis that occurs in neurodegenerative diseases and following acute ischemic episodes. Apoptosis is critical for the elimination of unwanted neurons within the developing nervous system. The Bcl-2 family of proteins contains pro- and a...

journal_title：Journal of bioenergetics and biomembranes

authors： Ward MW,Kögel D,Prehn JH

更新日期：2004-08-01 00:00:00

abstract：:Mitochondria are both targets and sources of oxidative stress. This dual relationship is particularly evident in experimental paradigms modeling ischemic brain injury. One mitochondrial metabolic enzyme that is particularly sensitive to oxidative inactivation is pyruvate dehydrogenase. This reaction is extremely impor...

journal_title：Journal of bioenergetics and biomembranes

authors： Fiskum G,Rosenthal RE,Vereczki V,Martin E,Hoffman GE,Chinopoulos C,Kowaltowski A

更新日期：2004-08-01 00:00:00

abstract：:Cyclosporin A (CsA) is highly neuroprotective in several animal models of acute neurological damage and neurodegenerative disease with inhibition of the mitochondrial permeability transition (mPT) having emerged as a possible mechanism for the observed neuroprotection. In the present study, we have evaluated two new n...

journal_title：Journal of bioenergetics and biomembranes

authors： Hansson MJ,Mattiasson G,Månsson R,Karlsson J,Keep MF,Waldmeier P,Ruegg UT,Dumont JM,Besseghir K,Elmér E

更新日期：2004-08-01 00:00:00

abstract：:We have studied a mutant strain of Drosophila in which 80% of the mitochondrial DNA molecules have lost over 30% of their coding region through deletion. This deletion affects genes encoding five subunits of complex I of the respiratory chain (NADH:ubiquinone oxidoreductase). The enzymatic activity of complex I in the...

journal_title：Journal of bioenergetics and biomembranes

authors： Farge G,Touraille S,Lachaume P,Debise R,Procaccio V,Alziari S

更新日期：2004-04-01 00:00:00

abstract：:Members of the genus Methanosarcina are strictly anaerobic archaea that derive their metabolic energy from the conversion of a restricted number of substrates to methane. H2 + CO2 are converted to CH4 via the CO2-reducing pathway, while methanol and methylamines are metabolized by the methylotrophic pathway. Two novel...

journal_title：Journal of bioenergetics and biomembranes

authors： Deppenmeier U

更新日期：2004-02-01 00:00:00

abstract：:The past 5 years has witnessed an advance in our understanding of alpha1G (Cav3.1), alpha1H (Cav3.2), and alpha11 (Car3.3), the pore-forming subunits of T-type or low-voltage-activated calcium channels (LVAs). LVAs differ in their localization and molecular, biophysical, and biochemical properties, but all conduct a t...

journal_title：Journal of bioenergetics and biomembranes

authors： Yunker AM,McEnery MW

更新日期：2003-12-01 00:00:00

abstract：:Complete initial steady state kinetics of NADH-decylubiquinone (DQ) oxidoreductase reaction between pH 6.5 and 9.0 show an ordered sequential mechanism in which the order of substrate bindings and product releases is NADH-DQ-DQH2-NAD+. NADH binding to the free enzyme is accelerated by protonation of an amino acid (pos...

journal_title：Journal of bioenergetics and biomembranes

authors： Hano N,Nakashima Y,Shinzawa-Itoh K,Terada H,Yoshikawa S

更新日期：2003-10-01 00:00:00

abstract：:Cytoplasmic organelles with an acidic luminal pH include vacuoles, coated vesicles, lysosomes, the Golgi apparatus, and synaptic vesicles. Acidic compartments are also known outside specialized cells such as osteoclasts. The unique acidic pH is formed by V-ATPase (Vacuolar type ATPase), other ion transporters, and the...

journal_title：Journal of bioenergetics and biomembranes

authors： Sun-Wada GH,Wada Y,Futai M

更新日期：2003-08-01 00:00:00

abstract：:Recent progress regarding the structure of the Ca(2+)-translocating ATPase of sarcoplasmic reticulum in several conformational states, and a substantial accumulation of biochemical information about this and other P-type ATPases, have put everything in place for the final convergence of biochemistry and structure that...

journal_title：Journal of bioenergetics and biomembranes

authors： Scarborough GA

更新日期：2003-06-01 00:00:00

abstract：:The soxL gene from Sulfolobus acidocaldarius (DSM 639) encodes a Rieske iron-sulfur protein. In this study we report the identification of two open reading frames in its downstream region. The first one, named soxN, codes for a membrane protein bearing a resemblance to the b-type cytochromes of the cytochrome bc1 and ...

journal_title：Journal of bioenergetics and biomembranes

authors： Hiller A,Henninger T,Schäfer G,Schmidt CL

更新日期：2003-04-01 00:00:00

abstract：:The role of nucleoside diphosphate (NDP) kinase with special reference to mammalian signal transduction systems was described. The interaction between NDP kinases and G proteins was reevaluated in view of their protein structural information and its significance was extended further on the basis of recent findings obt...

journal_title：Journal of bioenergetics and biomembranes

authors： Kimura N,Shimada N,Ishijima Y,Fukuda M,Takagi Y,Ishikawa N

更新日期：2003-02-01 00:00:00

abstract：:Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, non-climacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a...

journal_title：Journal of bioenergetics and biomembranes

authors： Almeida AM,Navet R,Jarmuszkiewicz W,Vercesi AE,Sluse-Goffart CM,Sluse FE

更新日期：2002-12-01 00:00:00

abstract：:Copper transporting P-type ATPases, designated ATP7A and ATP7B, play an essential role in mammalian copper balance. Impaired intestinal transport of copper, resulting from mutations in the ATP7A gene, lead to Menkes disease in humans. Defects in a similar gene, the copper transporting ATPase ATP7B, result in Wilson di...

journal_title：Journal of bioenergetics and biomembranes

authors： Cox DW,Moore SD

更新日期：2002-10-01 00:00:00

abstract：:The F1F0 complex of Paracoccus denitrificans (PdF1F0) is the fastest ATP synthase but the slowest ATPase. Sulfite exerts maximal activation of the PdF1F0-ATPase (Pacheco-Moisés, F., García, J. J., Rodríguez-Zavala, J. S., and Moreno-Sánchez, R. (2000). Eur J. Biochem. 267, 993-1000) but its effect on the PdF1F0-ATP sy...

journal_title：Journal of bioenergetics and biomembranes

authors： Pacheco-Moisés F,Minauro-Sanmiguel F,Bravo C,García JJ

更新日期：2002-08-01 00:00:00

abstract：:We have studied changes in plasma membrane NAD(P)H:quinone oxidoreductases of HL-60 cells under serum withdrawal conditions, as a model to analyze cell responses to oxidative stress. Highly enriched plasma membrane fractions were obtained from cell homogenates. A major part of NADH-quinone oxidoreductase in the plasma...

journal_title：Journal of bioenergetics and biomembranes

authors： Forthoffer N,Gómez-Díaz C,Bello RI,Burón MI,Martín SF,Rodríguez-Aguilera JC,Navas P,Villalba JM

更新日期：2002-06-01 00:00:00

abstract：:Arsenic trioxide is an effective treatment for acute promyelocytic leukemia, but resistance to metalloid salts is found in humans. Using atomic absorption spectroscopy, we have measured the rate of uptake of arsenic trioxide and of antimony tartrate in GLC4 and GLC4/ADR cells overexpressing MRP1 and the rate of their ...

journal_title：Journal of bioenergetics and biomembranes

authors： Salerno M,Petroutsa M,Garnier-Suillerot A

更新日期：2002-04-01 00:00:00

abstract：:We investigated the effects of K(ATP) channel openers diazoxide and pinacidil on the respiration rate and membrane potential (deltapsi) of rat heart mitochondria, oxidizing pyruvate and malate. Diazoxide and pinacidil (58.8-1348.3 microM) increased the V2 (-ADP) respiration rate accordingly by 13-208% and 30-273% and ...

journal_title：Journal of bioenergetics and biomembranes

authors： Kopustinskiene DM,Jovaisiene J,Liobikas J,Toleikis A

更新日期：2002-02-01 00:00:00

abstract：:P-glycoprotein (Pgp), the ATP-binding cassette (ABC) transporter, confers multidrug resistance to cancer cells by extruding cytotoxic natural product amphipathic drugs using the energy of ATP hydrolysis. Our studies are directed toward understanding the mechanism of action of Pgp and recent work deals with the assessm...

journal_title：Journal of bioenergetics and biomembranes

authors： Sauna ZE,Smith MM,Müller M,Kerr KM,Ambudkar SV

更新日期：2001-12-01 00:00:00

abstract：:Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with Vibrio fischeri, V. harveyi, and Photobacterium ...

journal_title：Journal of bioenergetics and biomembranes

authors： Bourgois JJ,Sluse FE,Baguet F,Mallefet J

更新日期：2001-08-01 00:00:00

abstract：:Proteins specifically involved in the biogenesis of respiratory complex I in eukaryotes have been characterized. The complex I intermediate associated proteins CIA30 and CIA84 are tightly bound to an assembly intermediate of the membrane arm. Like chaperones, they are involved in multiple rounds of membrane arm assemb...

journal_title：Journal of bioenergetics and biomembranes

authors： Schulte U

更新日期：2001-06-01 00:00:00

abstract：:Plasma membranes isolated from pig liver contained almost no acid sphingomyelinase but significant neutral magnesium-dependent sphingomyelinase that was activated by phosphatidylserine. We report here the purification to apparent homogeneity of neutral sphingomyelinase of about 87 kDa from liver plasma membranes. The ...

journal_title：Journal of bioenergetics and biomembranes

authors： Martín SF,Navarro F,Forthoffer N,Navas P,Villalba JM

更新日期：2001-04-01 00:00:00

abstract：:Mitochondrial porin facilitates the diffusion of small hydrophilic molecules across the mitochondrial outer membrane. Despite low sequence similarity among porins from different species, a "glycine-leucine-lysine" (GLK) motif is conserved in mitochondrial and Neisseria porins. To investigate the possible roles of thes...

journal_title：Journal of bioenergetics and biomembranes

authors： Runke G,Maier E,O'Neil JD,Benz R,Court DA

更新日期：2000-12-01 00:00:00

abstract：:In Propionigenium modestum, ATP is manufactured from ADP and phosphate by the enzyme ATP synthase using the free energy of an electrochemical gradient of Na+ ions. The P. modestum ATP synthase is a clear member of the family of F-type ATP synthases and the only major distinction is an extension of the coupling ion spe...

journal_title：Journal of bioenergetics and biomembranes

authors： Dimroth P,Matthey U,Kaim G

更新日期：2000-10-01 00:00:00

abstract：:The Mg2+ dependent asymmetry of the F(1)-ATPase catalytic sites leads to the differences in affinity for nucleotides and is an essential component of the binding-change mechanism. Changes in metal ligands during the catalytic cycle responsible for this asymmetry were characterized by vanadyl (V(IV) + O)2+, a functiona...

journal_title：Journal of bioenergetics and biomembranes

authors： Frasch WD

更新日期：2000-10-01 00:00:00

abstract：:The ATP synthase of the yeast Saccharomyces cerevisiae is composed of 20 different subunits whose primary structure is known. The organization of proteins that constitute the membranous domain is now under investigation. Cysteine insertions combined with the use of nonpermeant maleimide reagents and cross-linking reag...

journal_title：Journal of bioenergetics and biomembranes

authors： Velours J,Arselin G

更新日期：2000-08-01 00:00:00

abstract：:NM23s (or NDP kinases) regulate a fascinating variety of cellular activities, including proliferation, development, and differentiation. All these processes are modulated by external stimuli, leading to the idea that this family of proteins modulates transmembrane signaling pathways. This review summarizes the evidenc...

journal_title：Journal of bioenergetics and biomembranes

authors： Otero AS

更新日期：2000-06-01 00:00:00

abstract：:Hepatic iron toxicity because of iron overload seems to be mediated by lipid peroxidation of biological membranes and the associated organelle dysfunctions. However, the basic mechanisms underlying this process in vivo are still little understood. Gerbils were dosed with weekly injections of iron-dextran alone or in c...

journal_title：Journal of bioenergetics and biomembranes

authors： Masini A,Ceccarelli D,Giovannini F,Montosi G,Garuti C,Pietrangelo A

更新日期：2000-04-01 00:00:00

abstract：:Cellular Ca2+ signals are crucial in the control of most physiological processes, cell injury and programmed cell death; mitochondria play a pivotal role in the regulation of such cytosolic Ca2+ ([Ca2+]c) signals. Mitochondria are endowed with multiple Ca2+ transport mechanisms by which they take up and release Ca2+ a...

journal_title：Journal of bioenergetics and biomembranes

authors： Smaili SS,Hsu YT,Youle RJ,Russell JT

更新日期：2000-02-01 00:00:00

abstract：:This review is primarily focused on the contribution of our laboratory to study of the mitochondrial uncoupling UCPs. The initial stage was the description of a 32-kDa membranous protein specifically induced in brown adipose tissue mitochondria of cold-adapted rats. This protein was then shown by others to be responsi...

journal_title：Journal of bioenergetics and biomembranes

authors： Ricquier D,Miroux B,Cassard-Doulcier AM,Lévi-Meyrueis C,Gelly C,Raimbault S,Bouillaud F

更新日期：1999-10-01 00:00:00

abstract：:In the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and A...

journal_title：Journal of bioenergetics and biomembranes

authors： Costa AD,Nantes IL,Jezek P,Leite A,Arruda P,Vercesi AE

更新日期：1999-10-01 00:00:00

abstract：:Traditionally, mitochondria have been viewed as the "powerhouse" of the cell, i.e., the site of the oxidative phosphorylation machinery involved in ATP production. Consequently, much of the research conducted on mitochondria over the past 4 decades has focused on elucidating both those molecular events involved in ATP...

journal_title：Journal of bioenergetics and biomembranes

authors： Pedersen PL

更新日期：1999-08-01 00:00:00

abstract：:The unique bifurcated oxidation of ubiquinol at center P (Qo) of the cytochrome bc1 complex is the reaction within the Q-cycle reaction scheme that is most critical for the link between electron transfer and vectorial proton translocation. While there is a general consensus about the overall reaction at center P, the ...

journal_title：Journal of bioenergetics and biomembranes

authors： Brandt U

更新日期：1999-06-01 00:00:00